It increases the contrast of microscopic features in cells and tissues, which makes them easier to see when viewed through a microscope the word trichrome means three colours. Pdf comparison of three types of histochemical staining in. E tincion, hay dos variaciones principales utilizados. Como su nombre indica esta tincion emplea tres colorantes. Desparafinar xilol i, ii, iii cada uno por 5 min 2. Histologia tincion histology staining linkedin slideshare.
Staining differentiates tissues by tinting them in contrasting colours. Wistar rat, histology, hematoxylineosin staining, masson trichrome staining, pas. Ncleos negro citoplasma, queratina, fibras musculares y hemates rojo colgeno y reticulina azul o verde. Download as ppt, pdf, txt or read online from scribd. This page was last edited on 1 february 2019, at 09. Connective tissue can be selectively visualized by using a combination of three different staining solutions. Lavar durante dos minutos con solucion tampon ph 7,2.
All structured data from the file and property namespaces is available under the creative commons cc0 license. Massons trichrome is a threecolour staining protocol used in histology. Azophloxine and tungstophosphoric acid orange g solutions stain components such as muscle, cytoplasm and erythrocytes, and light green sf solution then is counter. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. As the name implies, three dyes are employed selectively staining muscle, collagen fibers, fibrin, and erythrocytes. Contratinte safranina, tine las celulas vegetativas. Stay connected to your students with prezi video, now in microsoft teams. Practica 3 tinciones bacterianas linkedin slideshare. Manual of histologic staining methods of the armed. Tecnologia medica mencion morfofisiopatologia y citodiagnostico.
Coloraciones tricromicas tincion tejido conectivo free. Files are available under licenses specified on their description page. Trichrome staining is a histological staining method that uses two or more acid dyes in conjunction with a polyacid. Used to differentiate between collagen and smooth muscle in tumors, and the increase of collagen in diseases such as cirrhosis.